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Novus Biologicals nbp2 01830
Nbp2 01830, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-dpp8+antibody+(oti1d2)/DPP8+Antibody+(OTI1D2)/pm37048172-61-22-23
Average 90 stars, based on 2 article reviews

nbp2 01830 - by Bioz Stars, 2026-09

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Article Title: DPP8 Selective Inhibitor Tominostat as a Novel and Broad-Spectrum Anticancer Agent against Hematological Malignancies
Article Snippet: This was followed by transfer to an Immobilon-P membrane (Millipore, Burlington, MA, USA) and hybridization with an anti-DPP8 antibody (OTI1D2), monoclonal, mouse, NBP2-01830 (Novus Biologicals, Englewood, CO, USA), an anti-DPP9 antibody (OTI2E3), monoclonal, mouse, NBP2-01521 (Novus Biologicals), an anti-GSDMD antibody, monoclonal, rabbit, ab210070 (Abcam, Cambridge, UK), an anti-Cleaved Caspase-3 antibody (Asp175, 5A1E), monoclonal, rabbit, #9664S (Cell Signaling Technology, Danvers, MA, USA), an anti-DFNA5/GSDME antibody (EPR19859, N-terminal), monoclonal, rabbit, ab215191 (Abcam), an anti-Caspase-1 antibody, polyclonal, rabbit, #2225S (Cell Signaling Technology), an anti-CARD8 antibody (2108C2a), monoclonal, mouse, sc-81213 (Santa Cruz Biotechnology, Dallas, TX, USA), an anti-Caspase-3 antibody, polyclonal, rabbit, #9662S (Cell Signaling Technology), an anti-HCK antibody (E1I7F), monoclonal, rabbit, #14643 (Cell Signaling Technology), an anti-LCK antibody (3A5), monoclonal, mouse, sc-433 (Santa Cruz Biotechnology), an anti-Fyn antibody (15), monoclonal, mouse, sc-434 (Santa Cruz Biotechnology), an anti-c-Fgr antibody (B-8), monoclonal, mouse, sc-166079 (Santa Cruz Biotechnology), an anti-Lyn antibody (H-6), monoclonal, mouse, sc-7274 (Santa Cruz Biotechnology), an anti-Blk antibody (9D10D1), monoclonal, mouse, sc-65980 (Santa Cruz Biotechnology), an anti-β-actin antibody (8H10D10), monoclonal, mouse, #3700S (Cell Signaling Technology), an anti-AK2 antibody, polyclonal, rabbit, ab37594 (Abcam), an anti-FADD antibody (A66-2), monoclonal, mouse, 556402 (BD Biosciences, Franklin Lakes, NJ, USA), an anti-phospho-FADD (Ser194) antibody, polyclonal, rabbit, #2781S (Cell Signaling Technology), or an anti-DUSP26 antibody, polyclonal, rabbit, GTX109283 (GeneTex, Zeeland, MI, USA).

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Article Title: DPP8 Selective Inhibitor Tominostat as a Novel and Broad-Spectrum Anticancer Agent against Hematological Malignancies.
Article Snippet: .. This was followed by transfer to an Immobilon-P membrane (Millipore, Burlington, MA, USA) and hybridization with an anti-DPP8 antibody (OTI1D2), monoclonal, mouse, NBP2-01830 (Novus Biologicals, Englewood, CO, USA), an anti-DPP9 antibody (OTI2E3), monoclonal, mouse, NBP2-01521 (Novus Biologicals), an anti-GSDMD antibody, monoclonal, rabbit, ab210070 (Abcam, Cambridge, UK), an anti-Cleaved Caspase-3 antibody (Asp175, 5A1E), monoclonal, rabbit, #9664S (Cell Signaling Technology, Danvers, MA, USA), an anti-DFNA5/GSDME antibody (EPR19859, N-terminal), monoclonal, rabbit, ab215191 (Abcam), an anti-Caspase-1 antibody, polyclonal, rabbit, #2225S (Cell Signaling Technology), an anti-CARD8 antibody (2108C2a), monoclonal, mouse, sc-81213 (Santa Cruz Biotechnology), an anti-Caspase-3 antibody, polyclonal, rabbit, #9662S (Cell Signaling Technology), an anti-HCK antibody (E1I7F), monoclonal, rabbit, #14643 (Cell Signaling Technology), an anti-LCK antibody (3A5), monoclonal, mouse, sc-433 (Santa Cruz Biotechnology), an anti-Fyn antibody (15), Cells 2023, 12, 1100 4 of 15 monoclonal, mouse, sc-434 (Santa Cruz Biotechnology, Dallas, TX, USA), an anti-c-Fgr antibody (B-8), monoclonal, mouse, sc-166079 (Santa Cruz Biotechnology), an anti-Lyn antibody (H-6), monoclonal, mouse, sc-7274 (Santa Cruz Biotechnology), an anti-Blk antibody (9D10D1), monoclonal, mouse, sc-65980 (Santa Cruz Biotechnology), an anti-β-actin antibody (8H10D10), monoclonal, mouse, #3700S (Cell Signaling Technology), an anti-AK2 antibody, polyclonal, rabbit, ab37594 (Abcam), an anti-FADD antibody (A66-2), monoclonal, mouse, 556402 (BD Biosciences, Franklin Lakes, NJ, USA), an anti-phospho-FADD (Ser194) antibody, polyclonal, rabbit, #2781S (Cell Signaling Technology), or an anti-DUSP26 antibody, polyclonal, rabbit, GTX109283 (GeneTex, Zeeland, MI, USA). .. Proteins detected by these antibodies were visualized with horseradish-peroxidase-conjugated anti-mouse or rabbit antibody (Santa Cruz Biotechnology) followed by the use of enhanced chemiluminescence (Amersham Pharmacia Biotech., Amersham, UK), as we described previously [16].

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DPP8-dependent antineoplastic effect of high-dose 1G244. ( A ) 1.0 × 10 5 of hematological cancer cell lines (MM.1S, KARPAS299, THP-1, KG1, Daudi, or NAMALWA) were cultured with DPP8/9 inhibitors (1G244 or talabostat) at doses of 0–100 µM for 72 h. Cell number was estimated by a colorimetric assay using WST-1 reagent (n = 6). ( B ) Knockdown studies of DPP8 and DPP9 in MM.1S or KARPAS299 cells. NT, no treatment; GFP, control vector; KD, knockdown. Expression level of DPP8 or DPP9 was estimated by Western blot analysis. ( C ) 1.0 × 10 5 of MM.1S and KARPAS299 cells and their transfectants were cultured with DPP8/9 inhibitors (1G244 or talabostat) at doses of 0–100 µM for 6 h. Cytotoxicity was estimated by a LDH release assay (n = 6).

Journal: Cells

Article Title: DPP8 Selective Inhibitor Tominostat as a Novel and Broad-Spectrum Anticancer Agent against Hematological Malignancies

doi: 10.3390/cells12071100

Figure Lengend Snippet: DPP8-dependent antineoplastic effect of high-dose 1G244. ( A ) 1.0 × 10 5 of hematological cancer cell lines (MM.1S, KARPAS299, THP-1, KG1, Daudi, or NAMALWA) were cultured with DPP8/9 inhibitors (1G244 or talabostat) at doses of 0–100 µM for 72 h. Cell number was estimated by a colorimetric assay using WST-1 reagent (n = 6). ( B ) Knockdown studies of DPP8 and DPP9 in MM.1S or KARPAS299 cells. NT, no treatment; GFP, control vector; KD, knockdown. Expression level of DPP8 or DPP9 was estimated by Western blot analysis. ( C ) 1.0 × 10 5 of MM.1S and KARPAS299 cells and their transfectants were cultured with DPP8/9 inhibitors (1G244 or talabostat) at doses of 0–100 µM for 6 h. Cytotoxicity was estimated by a LDH release assay (n = 6).

Article Snippet: This was followed by transfer to an Immobilon-P membrane (Millipore, Burlington, MA, USA) and hybridization with an anti-DPP8 antibody (OTI1D2), monoclonal, mouse, NBP2-01830 (Novus Biologicals, Englewood, CO, USA), an anti-DPP9 antibody (OTI2E3), monoclonal, mouse, NBP2-01521 (Novus Biologicals), an anti-GSDMD antibody, monoclonal, rabbit, ab210070 (Abcam, Cambridge, UK), an anti-Cleaved Caspase-3 antibody (Asp175, 5A1E), monoclonal, rabbit, #9664S (Cell Signaling Technology, Danvers, MA, USA), an anti-DFNA5/GSDME antibody (EPR19859, N-terminal), monoclonal, rabbit, ab215191 (Abcam), an anti-Caspase-1 antibody, polyclonal, rabbit, #2225S (Cell Signaling Technology), an anti-CARD8 antibody (2108C2a), monoclonal, mouse, sc-81213 (Santa Cruz Biotechnology, Dallas, TX, USA), an anti-Caspase-3 antibody, polyclonal, rabbit, #9662S (Cell Signaling Technology), an anti-HCK antibody (E1I7F), monoclonal, rabbit, #14643 (Cell Signaling Technology), an anti-LCK antibody (3A5), monoclonal, mouse, sc-433 (Santa Cruz Biotechnology), an anti-Fyn antibody (15), monoclonal, mouse, sc-434 (Santa Cruz Biotechnology), an anti-c-Fgr antibody (B-8), monoclonal, mouse, sc-166079 (Santa Cruz Biotechnology), an anti-Lyn antibody (H-6), monoclonal, mouse, sc-7274 (Santa Cruz Biotechnology), an anti-Blk antibody (9D10D1), monoclonal, mouse, sc-65980 (Santa Cruz Biotechnology), an anti-β-actin antibody (8H10D10), monoclonal, mouse, #3700S (Cell Signaling Technology), an anti-AK2 antibody, polyclonal, rabbit, ab37594 (Abcam), an anti-FADD antibody (A66-2), monoclonal, mouse, 556402 (BD Biosciences, Franklin Lakes, NJ, USA), an anti-phospho-FADD (Ser194) antibody, polyclonal, rabbit, #2781S (Cell Signaling Technology), or an anti-DUSP26 antibody, polyclonal, rabbit, GTX109283 (GeneTex, Zeeland, MI, USA).

Techniques: Cell Culture, Colorimetric Assay, Knockdown, Control, Plasmid Preparation, Expressing, Western Blot, Lactate Dehydrogenase Assay

Caspase-3-mediated apoptosis as anticancer effect by high-dose 1G244. ( A ) 1 × 10 6 MM.1S cells were cultured with DPP8/9 inhibitors (1G244 or talabostat) at doses of 0–100 µM for 3–48 h. The cleaved form (CL) of gasdermin-D (GSDMD), caspase-3, or gasdermin-E (GSDME) was detected by Western blot analysis. The upper caspase-3 CL: 19 kDa; and the lower caspase-3 CL: 17 kDa. ( B , C ) 1.0 × 10 5 of MM.1S cells were cultured with DPP8/9 inhibitors (1G244 or talabostat) at doses of 0–100 µM for 6 h with disulfiram (DSF), Z-DEVD, Z-VAD, necrostatin-1 (Nec), or necrosulfonamide (NSA). Cytotoxicity was estimated by a LDH release assay (n = 6).

Journal: Cells

Article Title: DPP8 Selective Inhibitor Tominostat as a Novel and Broad-Spectrum Anticancer Agent against Hematological Malignancies

doi: 10.3390/cells12071100

Figure Lengend Snippet: Caspase-3-mediated apoptosis as anticancer effect by high-dose 1G244. ( A ) 1 × 10 6 MM.1S cells were cultured with DPP8/9 inhibitors (1G244 or talabostat) at doses of 0–100 µM for 3–48 h. The cleaved form (CL) of gasdermin-D (GSDMD), caspase-3, or gasdermin-E (GSDME) was detected by Western blot analysis. The upper caspase-3 CL: 19 kDa; and the lower caspase-3 CL: 17 kDa. ( B , C ) 1.0 × 10 5 of MM.1S cells were cultured with DPP8/9 inhibitors (1G244 or talabostat) at doses of 0–100 µM for 6 h with disulfiram (DSF), Z-DEVD, Z-VAD, necrostatin-1 (Nec), or necrosulfonamide (NSA). Cytotoxicity was estimated by a LDH release assay (n = 6).

Techniques: Cell Culture, Western Blot, Lactate Dehydrogenase Assay

Dependence on HCK for DPP8/9 inhibitor-induced pyroptosis. ( A ) Expression level of DPP8, DPP9, caspase-1, CARD8, GSDMD, or caspase-3 in THP-1, MM.1S, KARPAS299, KG1, NAMALWA, or Daudi cells was estimated by Western blot analysis. ( B ) Gene expression of THP-1, MM.1S, KARPAS299, KG1, NAMALWA, or Daudi cells was analyzed by microarray method using 3D-Gene. ( C ) Expression level of HCK, LCK, Fyn, c-Fgr, Lyn, or Blk in THP-1, MM.1S, KARPAS299, KG1, NAMALWA, or Daudi cells was estimated by Western blot analysis. ( D ) 1.0 × 10 5 of hematological cancer cell lines (Jurkat, K562, MOLM-13, NOMO-1, RPMI8226, Raji, or SKM-1) were cultured with 1G244 at doses of 0–10 µM for 72 h. Cell number was estimated by a colorimetric assay using WST-1 reagent (n = 6). ( E ) Expression level of HCK or CARD8 in Jurkat, K562, MOLM-13, NOMO-1, RPMI8226, Raji, or SKM-1 cells was estimated by Western blot analysis. ( F ) Knockdown studies of HCK in MM.1S cells. NT, no treatment; GFP, control vector; KD, knockdown. Expression level of HCK was estimated by Western blot analysis. ( G ) 1.0 × 10 5 of MM.1S cells and their transfectants were cultured with DPP8/9 inhibitors (1G244 or talabostat) at doses of 0–100 µM for 6 h. Cytotoxicity was estimated by a LDH release assay (n = 6).

Journal: Cells

Article Title: DPP8 Selective Inhibitor Tominostat as a Novel and Broad-Spectrum Anticancer Agent against Hematological Malignancies

doi: 10.3390/cells12071100

Figure Lengend Snippet: Dependence on HCK for DPP8/9 inhibitor-induced pyroptosis. ( A ) Expression level of DPP8, DPP9, caspase-1, CARD8, GSDMD, or caspase-3 in THP-1, MM.1S, KARPAS299, KG1, NAMALWA, or Daudi cells was estimated by Western blot analysis. ( B ) Gene expression of THP-1, MM.1S, KARPAS299, KG1, NAMALWA, or Daudi cells was analyzed by microarray method using 3D-Gene. ( C ) Expression level of HCK, LCK, Fyn, c-Fgr, Lyn, or Blk in THP-1, MM.1S, KARPAS299, KG1, NAMALWA, or Daudi cells was estimated by Western blot analysis. ( D ) 1.0 × 10 5 of hematological cancer cell lines (Jurkat, K562, MOLM-13, NOMO-1, RPMI8226, Raji, or SKM-1) were cultured with 1G244 at doses of 0–10 µM for 72 h. Cell number was estimated by a colorimetric assay using WST-1 reagent (n = 6). ( E ) Expression level of HCK or CARD8 in Jurkat, K562, MOLM-13, NOMO-1, RPMI8226, Raji, or SKM-1 cells was estimated by Western blot analysis. ( F ) Knockdown studies of HCK in MM.1S cells. NT, no treatment; GFP, control vector; KD, knockdown. Expression level of HCK was estimated by Western blot analysis. ( G ) 1.0 × 10 5 of MM.1S cells and their transfectants were cultured with DPP8/9 inhibitors (1G244 or talabostat) at doses of 0–100 µM for 6 h. Cytotoxicity was estimated by a LDH release assay (n = 6).

Techniques: Expressing, Western Blot, Gene Expression, Microarray, Cell Culture, Colorimetric Assay, Knockdown, Control, Plasmid Preparation, Lactate Dehydrogenase Assay

Antitumor effects of tominostat. ( A ) 1.0 × 10 5 of MM.1S, KARPAS299, or Daudi cells were cultured with DPP8/9 inhibitors (1G244 or tominostat) at doses of 0–100 µM for 6 h. Cytotoxicity was estimated by a LDH release assay (n = 6). ( B , C ) 5 × 10 6 of MM.1S ( B ) or Daudi ( C ) cells were subcutaneously inoculated into NSG mice (n = 6). Three days after inoculation, 1G244 or tominostat was administered subcutaneously once a week. Tumor volume was assessed at the same time.

Journal: Cells

Article Title: DPP8 Selective Inhibitor Tominostat as a Novel and Broad-Spectrum Anticancer Agent against Hematological Malignancies

doi: 10.3390/cells12071100

Figure Lengend Snippet: Antitumor effects of tominostat. ( A ) 1.0 × 10 5 of MM.1S, KARPAS299, or Daudi cells were cultured with DPP8/9 inhibitors (1G244 or tominostat) at doses of 0–100 µM for 6 h. Cytotoxicity was estimated by a LDH release assay (n = 6). ( B , C ) 5 × 10 6 of MM.1S ( B ) or Daudi ( C ) cells were subcutaneously inoculated into NSG mice (n = 6). Three days after inoculation, 1G244 or tominostat was administered subcutaneously once a week. Tumor volume was assessed at the same time.

Techniques: Cell Culture, Lactate Dehydrogenase Assay

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